For such small amounts of sample, we recommend using a Vibratory Micro Mill PULVERISETTE 0. Also the usage of a Planetary Micro Mill PULVERISETTE 7 classic line is possible to achieve way finer results.
mortar and 50 mm Ø grinding ball made of sintered corundum (Al2O3)
Feed quantity:
0.3 g
Feed Size:
3-4 cm
Grinding time:
30 min
Final fineness:
< 500 µm
Comments:
After 30 minutes of dry grinding, sample starts sticking stronger to bowl and balls.
This uses to happen after the majority of particles reached a fineness of < 20-30 µm. Interacting forces between fine ground particles will become bigger as their own g-force. Therefore, particles will stick to each other and become compressed by the used grinding balls. These clusters of particles also contain bigger particles which will not be ground any further.
A longer dry grinding is not possible. Still pieces of hear with a length up to 500 µm can be found.
Hair uses to be flexible and ductile; for this, a longer grinding time is expected to be needed.
It took about 20 minutes until a sticking occurred with the Planetary Micro Mill PULVERISETTE 7 premium line. Afterwards, the fineness of ground sample has been checked by optical microscopy. We are still able to find particles up to ~ 100 µm.
Longer dry grinding time is not possible. To eliminate sticking effects, we recommend proceed grinding in suspension.