The material (20 leaf) was careful transfered with a brush in to the grinding bowl.
Avolan and isopropanole were used as tensides, because the sample is very difficult to get sprinkle.
The grinding time is compound of : 5min grinding + 10min cooling x 24-repetitions. Because the grinding set is coated after the grinding, we recommend, to fill the grinding set with sand and water, and run a cleaning cyclus for 5min.