MENU - PARTNER AREA

pine needles

Protocol number:	M0099139
Industry:	Biology
Feed Size:	1 – 2 cm
Desired Fineness:	fine powder
Quantity:	1 g
Recommendation:	For a metal free grinding of the soft pine tree needles, we recommend using a Vibratory Micro Mill PULVERISETTE 0 to grind the sample after embrittlement with liquid nitrogen.

Result 1

	Vibratory Micro Mill PULVERISETTE 0 amplitude: 2.5 mm
Equipment:	mortar: zirconium oxide + ball: 50 mm Ø zirconium oxide + Cryo-Box
Feed quantity:	1.0 g
Feed Size:	1 cm
Additive:	+ liquid nitrogen
Grinding time:	10 min
Final fineness:	fine powder
Comments:	Sample needs to be dry and brittle for a proper dry grinding with a ball mill. The pine needle sample use to be too soft for a grinding without liquid nitrogen. For this, we placed the sample and mortar into the optional Cryo-Box and pored liquid nitrogen over for embrittlement. After sample, mortar and grinding ball have been chilled, we started the grinding process. Evaporated nitrogen has been refilled during the grinding process. After 10 minutes of grinding, the pine needles became ground to a fine powder. After the remaining nitrogen has been evaporated, cold sample has been packed and sealed to avoid a condensation of moisture until room temperature has been reached.