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bones (dried - beef)

Protocol number:	M0099057
Industry:	Biology, Agriculture and forestry
Feed Size:	< 3 cm
Desired Fineness:	< 10 mm
Quantity:	25-150 g
Recommendation:	We recommend using a Power Cutting Mill PULVERISETTE 25 for comminution of sample.

Result 1

	Cutting Mill PULVERISETTE 19 large speed 50-700 rpm + frequency converter: rotation speed 150 rpm (standard 300 rpm) + knives adjusted to the max. gap 3 - 4 mm
Equipment:	rotor with V-cutting edges and fixed knives made of hardmetal tungsten carbide (WC) + sieve cassette 10,0 mm square perforation
Feed quantity:	25 g
Feed Size:	3x3x1 cm
Grinding time:	1 min
Final fineness:	50.8% = 4-10 mm 33.9% < 2 mm
Comments:	To produce the least amount of fine particles, we used a frequency converter for a further reduction of rotor speed. On request, different gear boxes use to be available for a cutting mill. We have chosen a wider gap between knives of rotor and fixed knives in the grinding chamber to minimize formation of fine particle. After grinding, still a few particles use to be left loose on top of the sieve cassette. The more sample will be fed, the less will be the loss (w%) of those particles which will not been grabbed by the rotor. Cleaning of the grinding chamber can be performed rapidly by vacuuming with a soft brush. Within 1 minutes, the bone disks have been comminuted. To determine resulting particles, we sieved out the complete sample. We found 50,8 % 4-10 mm fraction and 33,9 % < 2 mm fraction.